Fig. 2
From: Nanodiamond enhances immune responses in mice against recombinant HA/H7N9 protein
Purification and characterizations of recombinant trimeric H7 expressed in N. benthamiana leaves. a Recombinant H7-pII produced from agro-infiltrated tobacco leaves and detected by Western blot: H7-pII: 20 µg of total soluble protein extracts from tobacco leaves; Wt: wild type used as the negative control. b Purification of H7-pII from total soluble protein extracts using IMAC: RE raw extract, FT flow through, W wash fraction, P purified fraction in SDS-PAGE (Left) and H7-pII immunological detection via anti-c-myc monoclonal antibody (Right); WT wild type N. benthamiana; M protein marker (Right). c Detection of oligomeric states of H7-pII via the cross-linking reaction: (−) and (+) BS3: indicate a cross-linker bis(sulfosuccinimidyl)substrate (BS3) with 0 and 5 mM final concentration, respectively. The resulting products were separated via the 4–10% gradient SDS-PAGE under reducing conditions, blotted and detected via anti-c-myc monoclonal antibody