Fig. 4
CD20 Ab-mPEG scFv enhances PLD anti-proliferative activity. a Raji cells were incubated with CD20 Ab-mPEG scFv (red squares) or CD20 Ab-DNS scFv (green triangles) for 20 min followed by addition of serial dilutions of PEGylated liposomal doxorubicin (PLD) in triplicate for 3 h. The cells were also incubated with culture medium for 20 min followed by addition of serial dilutions of PLD (blue circles). Cell viability was measured with ATPlite Luminescence Assay System after 72 h of incubation. b The half maximal effective concentration (EC50) values of Raji cells treated with CD20 Ab-mPEG scFv plus PLD, CD20 Ab-DNS scFv plus PLD or PLD alone were analyzed. Data are shown as mean ± s.d. PEG BsAb, CD20 Ab-mPEG scFv; DNS BsAb, CD20 Ab-DNS scFv; PLD, PEGylated liposomal doxorubicin