Fig. 6
From: Decrease in membrane fluidity and traction force induced by silica-coated magnetic nanoparticles
reproduced from our previous study, Copyright © 2019 Springer Nature [37]
Metabotranscriptomic analysis of the microarray and metabolite profile of MNPs@SiO2(RITC)-treated cells. a Lipid peroxidation and focal adhesion-related genes and metabolite networks were algorithmically constructed using IPA. Red and green areas indicate up- and downregulated genes, respectively. Lines indicate indirect (dotted) or direct (solid) relationship. Differentially expressed genes obtained from microarray data (> threefold change) and disturbances in the metabolic profile (> 20% change) are shown. Quantitative evaluation of the metabotranscriptomic network-related genes by b semi-quantitative reverse transcription (RT)-PCR and c quantitative real-time PCR. Cells were treated with 0.1 and 1.0 μg/µL MNPs@SiO2(RITC) for 12 h. RT-PCR and qPCR were performed using gene-specific primer pairs for SOD2, LIMS1, NCK1, and C3AR1. GAPDH was used as the internal control. PCR products were normalised relative to the levels of internal control. Data represent the mean ± SD of 3 independent experiments. *p < 0.05 vs untreated control. Transcriptome and metabolome data are