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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: GSK-3β suppression upregulates Gli1 to alleviate osteogenesis inhibition in titanium nanoparticle-induced osteolysis

Fig. 2

Ti particle intervention suppressed Hh-Gli1 signaling in MC3T3-E1 cells. A Gli1 mRNA levels. B Protein levels of Gli1 after intervention. C Protein levels of Gli1 in the cytoplasm and nucleus after intervention. D Representative images of immunofluorescence staining; red (phalloidin), green (Gli1), and blue (nuclei). E Quantitative analysis of nucleus Gli1 protein ratio. F Quantitative analysis of the average fluorescence intensity of Gli1. All groups except the control group were cultured in osteogenic medium. n = 3 per group. *p < 0.05, **p < 0.01, ns: no significance

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