Fig. 5
![Fig. 5](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12951-022-01387-9/MediaObjects/12951_2022_1387_Fig5_HTML.png)
SHED encapsulated in hydrogel was induced to differentiate and detect neuron-related markers. A The results of immunofluorescence analysis showed the expression of Nestin markers; B Fluorescence intensity statistics of Nestin (FITC). C The results of immunofluorescence analysis showed the expression of GFAP. D Fluorescence intensity statistics of Nestin (FITC); E qPCR was used to detect the gene expression level of Nestin; F The mRNA level of GFAP was analyzed. One-way ANOVA was used for statistical significance. The data was shown in mean ± SEM. ***p < 0.001, **p < 0.01, *p < 0.05. Three independent experiment repetitions were performed