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Fig. 2 | Journal of Nanobiotechnology

Fig. 2

From: Highly potent multivalent VHH antibodies against Chikungunya isolated from an alpaca naïve phage display library

Fig. 2

Fabrication, characterization and binding activity detection of nanobodies. a Schematic representation of the expression vector pET-SUMO for nanobody. b Soluble prokaryotic expression and purification of recombinant nanobody using an N-terminally 6 × His- and SUMO-tagged protein (~ 28 kDa).c Binding of SUMO-tagged VHHs to immobilized CHIKV E2 (~ 40kD) or Chikungunya virion was evaluated by western blot. d Binding of SUMO-tagged VHHs to immobilized CHIKV E2 or CHIKV virus was quantified by indirect ELISA. e Particle size distribution of SUMO-tagged VHHs by NTA. f Negative-stain TEM images of SUMO-tagged VHHs, Scale bar, 50 nm

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Journal of Nanobiotechnology

ISSN: 1477-3155

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