Fig. 3
Inflammatory cytokine binding ability of Neu-LPs in vitro. A Neutralization dose response of proinflammatory factors (TNFα, IL1β, IL6, and CXCL2) by Neu-LPs. *P < 0.05 and ***P < 0.001 compared with the 0 mg/ml group. B Binding capacity of Neu-LPs to inflammatory cytokines (TNFα, IL1β, IL6, and CXCL2). IC50 values were derived from the variable slope model using GraphPad Prism 7. C Schematic illustration of a direct co-culture of macrophages and cardiomyocytes. D Expression levels of proinflammatory cytokines (TNFα, IL1β, IL6, and CXCL2) in the in vitro myocardial inflammation model with or without Neu-LP treatment. Statistical analysis was performed using one-way ANOVA. Data are presented as mean ± SD. **P < 0.01 and ***P < 0.001 compared with the NC group