Fig. 2

Preparation and characterization of nanoICG. A Schematic of nanoICG preparation via SPFT for clinical diagnosis and treatment. B, C mixture due to the pelleted Representative SEM images of nanoICG, scale bar: 1 μm (B) and 500 nm (C). D DLS of nanoICG. E The molecule structure of LC-MS of freeICG and nanoICG. F, G The fluorescence intensities of nanoICG in different pH conditions were detected by IVIS Lunima LT at the 745 nm excitation after 1 h incubation. H Fluorescence intensity of freeICG and nanoICG was detected at different times by a 745 nm excitation when pH is 6.5. Data represent mean ± SD, n = 3. * P < 0.05, **** P < 0.0001, Student’s t-test