Fig. 5
![Fig. 5](http://media.springernature.com/full/springer-static/image/art%3A10.1186%2Fs12951-022-01532-4/MediaObjects/12951_2022_1532_Fig5_HTML.png)
Biosafety evaluation of BMP-Ag and Ag-Fe3O4 NPs. Cytotoxicity of BMP-Ag and Ag-Fe3O4 NPs compared with Ag and BMP in different cell lines: A L929 and B LO2. The cell viability of LO2 and L929 was higher than 75% at the highest concentration of 1000 μg mL−1 of BMP-Ag and Ag-Fe3O4 NPs, which exhibited lower cell cytotoxicity. C Fluorescence microscopy images of LO2 and L929 stained with the Calcein-AM/PI kit after treatment with Ag-Fe3O4, BMP-Ag, Ag, and BMP NPs at the highest concentration 1000 μg mL−1