Fig. 2From: Controllable and reusable seesaw circuit based on nicking endonucleasesSchematic illustration of the enzymes’ function, including nicking endonuclease Nt.BbvCI, trypsin, and trypsin inhibitor. (A) Schematic illustration of the activity of nicking endonuclease, trypsin, and trypsin inhibitor; (B) Fluorescence curves of experiments demonstrating the activity of nicking endonuclease and trypsin; (C) Fluorescence curves of experiments with various concentrations of trypsin; (D) Fluorescence curves of experiments demonstrating the activity of trypsin inhibitor; (E) Fluorescence curves of experiments with various concentrations of trypsin inhibitors; (F) Experimental process and (G) Fluorescence curves of experiments demonstrating the breakthrough of trypsin inhibitors. Ordaining fluorescent results of fluorescently FAM-labeled strand as 100% and Reporter duplex as 0%. The nucleic acid sequence and concentration of this part’s experiments are shown in Table S1Back to article page