Fig. 6
Exosomal miR-9-5p in iPSC-MSC-EXOs inhibited cardiomyocyte mitochondrial fission by inhibiting VPO1/ERK signaling pathway. A Bioinformatics analysis predicted the binding sites between VPO1 and miR-9-5p. B Luciferase reporter assays confirmed the association of VPO1 and miR-9-5p. C qRT-PCR analysis of the level of miR-9-5p in NMCMs treated with control, miR-9-5p mimic or miR-9-5p inhibitor. D qRT-PCR analysis of the level of VPO1 in NMCMs treated with control, miR-9-5p mimic or miR-9-5p inhibitor. E Western blotting analysis of the level of VPO1 in NMCMs treated with control, miR-9-5p mimic or miR-9-5p inhibitor. F Western blotting analysis of the level of VPO1, p-Drp1, Drp1, p-ERK, ERK in control, DOX, DOX + iPSC-MSC-EXOs, DOX + miR-9-5pKD-iPSC-MSC-EXOs and DOX + miR-9-5pKD-iPSC-MSC-EXOs + U0126 treated NMCMs. n = 3 biological replicates for each group. Data are expressed as mean ± SD. ***p < 0.001, ns = not significant