Fig. 7

Effect of cBMDM-sEVs on proliferation and inflammation of synovium in vivo and in vitro by targeting mTOR and downstream signal pathway. (A) Venn diagram of the targeting genes of miR-100-5p predicted by TargetScan, miRDB, and microT. (B) mRNA expression of mTOR in RA-FLS cocultured with PBS, cBMDM-sEVs or nBMDM-sEVs, respectively. (C) mRNA expression of mTOR in RA-FLS cocultured with different concentrations of cBMDM-sEVs. (D) Representative fluorescence images of mTOR in RA-FLS cocultured with PBS, cBMDM-sEVs or nBMDM-sEVs. (E) mTOR expression in knee and ankle joints analyzed by IHC. (F-G) Protein expression of mTOR, p-mTOR, p70s6K, p-p70s6K, 4E-BP1, and p-4E-BP1 by WB. Data are presented as mean ± SD, *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001