Fig. 5
Ferroptosis parameters evaluated on tumor cells. a-c, CLSM images (a), C11-BODIPY581/591 (oxidized form) fluorescence distributions (b) and quantitative analysis of the C11-BODIPY581/591 (oxidized form) fluorescence intensities measured by flow cytometry (c) of the 4T1 cells after treatment with PBS (I), MSPION3 (II), SFN (III), BQR (IV), SFN + BQR (V), SFN1@MSPION3 (VI), BQR1@MSPION3 (VII), or SFN/BQR1@MSPION3 (VIII) for 24 h, and staining with C11-BODIPY581/591. The observed increase in green fluorescence (oxidized form) and decrease in red fluorescence (reduced form) indicate the generation of lipid peroxidation (LPO). d-f, CLSM images (d), green fluorescence distributions (e), and quantitative analysis of the DIO fluorescence intensities measured by flow cytometry (f) of 4T1 cells after different treatments for 24 h, showing the structural integrity of the cell membrane