Fig. 1
Characterization of MVMαCD3chDC. A The protein amount fold change of EMchDC and MVMchDC from a constant number of chDC. B The PD-L1 mean fluorescence intensity of chDC, EMchDC and MVMchDC were determined by flow cytometry analysis. CEx vivo tissue distribution of EMchDC and MVMchDC at 24 h after tail vein injection. EMchDC and MVMchDC were labeled using DiD fluorescence. D Quantitative analysis of EMchDC and MVMchDC in heart, liver, spleen, lung, and kidney. E The radiance ratio of spleen to liver for EMchDC and MVMchDC in mice. F Image of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) protein analysis. G-H Percentage of azido-labeled chDC and MVMchDC after different concentration of Ac4ManNAz treatments. chDC were treated with different concentrations of Ac4ManNAz for 3 days. I Percentage of αCD3-positive MVMAzidochDC after 2 h co-culture with DBCO-αCD3. Grey indicated control fluorescence in MVMAzidochDC that were not incubated with DBCO-αCD3. J TEM image of MVMαCD3chDC. Scale bar = 200 nm. K-L Hydrodynamic diameter and ζ-potential of MVMimDC, MVMchDC and MVMαCD3chDC. M-N αCD3 antibodies stability and size stability of MVMαCD3chDC in PBS with serum for 5 days. Data were presented as mean ± SD. Statistical analyses were performed by Student’s t-test. N = 3 per group. ns P > 0.05, *P < 0.05, and **P < 0.01