Fig. 3

MVM^αCD3_chDC promoted Treg polarization of T cells and M2 polarization of macrophages in vitro. A Percentage of the CD3⁺CD4⁺FoxP3⁺ Treg in CD3⁺CD4⁺ T cells using flow cytometry analysis. B Relative mRNA expression of FoxP3 and IL-10 of splenic lymphocytes after different treatments. C The concentration of IL-10 in supernatant of splenic lymphocytes after different treatments. D Percentage of F4/80⁺CD86⁺ M1 and F4/80⁺CD206⁺ M2 in F4/80⁺ macrophages and the corresponding M2/M1 ratio using flow cytometry analysis. E Relative mRNA expression of iNOS and TNF-α of M1 macrophage after different treatments. F The concentration of TNF-α in supernatant of M1 macrophages after different treatments. G Relative mRNA expression of ARG-1 and TGF-β of M2 macrophages after different treatments. H The concentration of TGF-β in supernatant of M2 macrophages after different treatments. Data were presented as mean ± SD. Statistical analyses were performed by One-way ANOVA with Tukey method. N = 3 per group. ns P > 0.05, *P < 0.05, **P < 0.01, ***P < 0.001, and ****P < 0.0001