Fig. 5
In vitro PTT with CY7-4. A Viability of L-O2 cells, HUVECs, and HK2 cells treated with different concentrations of CY7-4 for 12 h. B Viability of P69 and RM-1 cells treated with various concentrations of CY7-4 for 12 h with or without 808 nm laser irradiation (1 W cm−2, 5 min). C Fluorescence images of RM-1 cells treated with 20 μM CY7-4 or ICG with 808 nm laser irradiation and calcein-AM/PI costaining; laser irradiation alone served as a negative control (1 W cm−2, 5 min). Red channel: dead cells; green channel: living cells; scale bar: 200 μm. D Fluorescence images of RM-1 cells treated with 20 μM CY7-4 for 30 min (808 nm laser irradiation, 1 W cm−2, 5 min) stained with TUNEL. The DNase I-treated group served as a positive control. Scale bar: 20 μm