Fig. 5

Migrasomes promote adipose tissue regeneration. (A) Schematic illustration of injection of poorly vascularized Ischemic adipose tissue with migrasomes (+ Mig group) or PBS (PBS group). N = 7 in each groups. (B) Western blot analysis of CXCL12 protein expression in adipose tissue from the + Mig and PBS groups at 7 days after surgery. (C) Semiquantitative analysis of CXCL12 protein expression. *p < 0.05 compared with PBS group. (D) Macroscopic image of adipose tissue from the + Mig and PBS groups at 30 days after surgery. Scale bar, 50 mm. (E) HE staining of adipose tissue from the + Mig and PBS groups at 30 days after surgery. Scale bar, 200 μm. (F) Immunofluorescence staining of CD34 and perilipin in adipose tissue from the + Mig and PBS groups at 30 days after surgery. Scale bar, 20 μm. (G) Quantitative analysis of infiltrated CD34⁺ ASCs per field in adipose tissue from the + Mig and PBS groups over time. (H) Quantitative analysis of the perilipin⁺ area per field in adipose tissue from the + Mig and PBS groups over time. *p < 0.05 compared with PBS group. (I) Western blot analysis of CXCR4, RhoA, and PPARγ protein expression in adipose tissue from the + Mig and PBS groups 7 days after surgery. (J-L) Semiquantitative analysis of CXCR4, RhoA, and PPARγ protein expression. *p < 0.05 compared with PBS group. The data are mean ± SEM. Statistical differences in (C) were tested by nonparametric Mann-Whitney test. (G), (H) and (J) to (L) were analyzed by One-way ANOVA